Brazilian Journal of Pulmonology

ISSN (on-line): 1806-3756 | ISSN (printed): 1806-3713

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Experimental model of isolated lung perfusion in rats: technique and application in lung preservation studies

Modelo experimental de perfusão pulmonar isolada em ratos: técnica e aplicações em estudos de preservação pulmonar

Paulo Manuel Pêgo-Fernandes, Eduardo de Campos Werebe, Paulo Francisco Guerreiro Cardoso, Rogério Pazetti, Karina Andrighetti de Oliveira, Paula Roberta Otaviano Soares, Fabio Biscegli Jatene

J Bras Pneumol.2010;36(4):-

Abstract PDF PT PDF EN Portuguese Text

Small animal models are particularly suitable for lung preservation studies, because they are simple and cost-effective. This brief communication focuses on the technical description of an ex vivo lung perfusion model in rats by means of a commercially available apparatus, which was the first to be installed in a thoracic surgery research laboratory in Brazil. The model and its preparation, together with its applications for lung preservation studies, are described in detail. All technical details can also be seen in a video posted on the website of the Brazilian Journal of Pulmonology.

 


Keywords: Lung transplantation; Reperfusion injury; Rats; Models, animal.

 


Ex vivo lung perfusion: initial Brazilian experience

Perfusão pulmonar ex vivo: experiência nacional inicial

Paulo Manuel Pêgo-Fernandes, Israel Lopes de Medeiros, Alessandro Wasum Mariani, Flávio Guimarães Fernandes, Fernando do Valle Unterpertinger, Marcos Naoyuki Samano, Eduardo de Campos Werebe, Fábio Biscegli Jatene

J Bras Pneumol.2009;35(11):1107-1111

Abstract PDF PT PDF EN Portuguese Text

In the last 20 years, lung transplantation has become the standard treatment for patients with end-stage lung disease. However, less than 20% of the donor lungs available for transplant are actually usable. This disparity between the growing number of recipients and the small number of donors has resulted in increased mortality among lung transplant candidates on waiting lists. Strategies such as the utilization of organs from marginal donors have proven ineffective in increasing the number of transplants. In 2000, a new method for reconditioning human lungs that had been previously rejected for transplantation was developed in Sweden. We describe our initial experience with ex vivo lung perfusion.

 


Keywords: Lung transplantation; Organ preservation; Organ preservation solutions; Thoracic surgery.

 


Questionnaire of quality of life in patients with primary hyperhidrosis

Questionário de qualidade de vida em pacientes com hiperidrose primária

José Ribas Milanez de Campos, Paulo Kauffman, Eduardo de Campos Werebe, Laert Oliveira Andrade Filho, Sergio Kuzniek, Nelson Wolosker, Fábio Biscegli Jatene, Mariane Amir

J Bras Pneumol.2003;29(4):178-181

Abstract PDF PT

Background: Hyperhidrosis or excessive sudoresis is a chronic disease associated with important subjective distress. Objective: To propose a specific questionnaire to evaluate the quality of life of patients with hyperhidrosis. Methods: From October 1995 to March 2002, 378 patients (234 females), with a mean age of 26.8 years, were evaluated before and after video-assisted thoracic sympathectomy. Results: Therapeutic success was obtained in 90% of the procedures. The recurrence rate was 10% for palmar and 11% for axillary hyperhidrosis; 27% of the patients who had recurrence were re-operated successfully. No serious complications were reported. Of the total number of patients, 91% answered to the quality of life questionnaire, and 86% of them reported improvement after the procedure. Conclusions: Thoracic sympathectomy is a therapeutic method capable of changing the quality of life of patients with hyperhidrosis. The questionnaire applied has shown these changes.

 


Keywords: Thoracic sympathectomy. Quality of life.

 


 

 


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CNPq, Capes, Ministério da Educação, Ministério da Ciência e Tecnologia, Governo Federal, Brasil, País Rico é País sem Pobreza
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